Home | About us | Editorial board | Ahead of print | Current issue | Archives | Search | Submit article | Instructions | Subscribe | Advertise | Contact us |  Login 
National Journal of Maxillofacial Surgery
 
Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 823
 


 
Table of Contents
CASE REPORT
Year : 2014  |  Volume : 5  |  Issue : 2  |  Page : 195-197  

Epithelial-myoepithelial carcinoma of floor of mouth: A case report with cytological, histological and immunohistochemical correlation


Department of Oral and Maxillofacial Surgery, Maulana Azad Institute of Dental Sciences, New Delhi, India

Date of Web Publication10-Apr-2015

Correspondence Address:
Sujata Mohanty
Department of Oral and Maxillofacial Surgery, Maulana Azad Institute of Dental Sciences, Bahadur Shah Zafar Marg, New Delhi - 110 002
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-5950.154835

Rights and Permissions
   Abstract 

A 61-year-old female presented with a 3-year-old swelling in the right floor of mouth. Clinical examination and fine needle aspiration cytology suggested a benign lesion. The mass was excised locally along with the involved sublingual and deep part of submandibular gland and duct. Post-operative histopathological examination revealed features of pleomorphic adenoma. However, on revision of histological sections, features were predominantly of a rare malignancy of the salivary glands, epithelial-myoepithelial carcinoma (EMC), along with focal areas of adenoid cystic carcinoma (Ad CC). The tumor was p-63, s-100 and smooth muscle actin positive but C-kit was negative, which ruled out Ad CC and the possibility of a hybrid carcinoma. The aim of this article is to describe a rare case of EMC in the floor of mouth and the confusing cytological picture that it created.

Keywords: Salivary gland tumor, sublingual gland, submandibular gland


How to cite this article:
Mohanty S, Pathak H. Epithelial-myoepithelial carcinoma of floor of mouth: A case report with cytological, histological and immunohistochemical correlation. Natl J Maxillofac Surg 2014;5:195-7

How to cite this URL:
Mohanty S, Pathak H. Epithelial-myoepithelial carcinoma of floor of mouth: A case report with cytological, histological and immunohistochemical correlation. Natl J Maxillofac Surg [serial online] 2014 [cited 2019 Nov 20];5:195-7. Available from: http://www.njms.in/text.asp?2014/5/2/195/154835


   Introduction Top


Epithelial-myoepithelial carcinoma (EMC) is a rare biphasic low grade malignancy accounting for only 0.5% of all salivary gland tumors. [1] There have been reports suggesting co-existence of another malignancy, like adenoid cystic carcinoma (Ad CC) with EMC, in the same tumor. Such tumors are named "hybrid carcinomas." [2] If EMC develops in a long standing case of pleomorphic adenoma (PA), it is called EMC-ex PA. [3] Ours is a case of a tumor in the submandibular and sublingual salivary glands diagnosed as EMC, having clinical and histological resemblance to cellular PA.


   Case Report Top


The present case report is about a 61-year-old woman who reported to us with a growth in the floor of mouth that gradually increased in size since last 3 years, without pain. On examination, a 3 cm × 2.5 cm ovoid swelling was palpated lingual to the edentulous alveolar ridge, extending from midline (lingual frenum) to the right premolars [Figure 1]. It was a firm, non-tender, non-compressible, non-reducible, did not move with deglutition and showed no change in size with meals. No pulsations were felt over the mass and no vascular channels seen. The swelling was not fixed to underlying tissues and salivary secretions were normal. Some cervical lymph nodes were palpable but not tender. There was no neural involvement or sudden increase in size. Complete hemogram, serum electrolyte levels, kidney function test and liver function test had all values within the normal range, but for a raised erythrocyte sedimentation rate (50 mm/h). Fine needle aspiration cytology (FNAC) of the lesion suggested PA. Node Ib was palpable, but non-reactive on FNAC. Magnetic resonance imaging revealed a 20 mm × 18 mm × 15 mm well defined well-marginated lesion in right sublingual space in the region of anterior aspect of sublingual gland [Figure 2]. It indented the inferior surface of tongue and right genioglossus muscle and did not cross midline. Multiple mildly enlarged bilateral level Ib, II and III lymph nodes were noted. A provisional diagnosis of PA of sublingual gland was made and local excision of the lesion along with the gland was planned under L.A. Incision was placed from midline extending posteriorly near the alveolar ridge in order to protect the Wharton's duct. With careful dissection, lingual nerve was identified and protected. On surgical exploration, it was found that the mass involved the deep part of submandibular gland and duct with the sublingual gland being compressed by the mass. It was difficult to delineate the exact origin of the pathology (sublingual or submandibular gland); hence it was decided to sacrifice the sublingual gland, deep part of submandibular gland and its duct on the affected side [Figure 1]. Excision was done with a cuff of healthy overlying mucosa. Neck dissection was not indicated, hence not done. Initial histopathological examination, revealed a solid tumor tissue composed of myoepithelial and ductal cells presenting as double layered duct like structures with hyalinization of stroma. The epithelial component showed diversity in the form of sheets, nests and cords with different patterns of ductal arrangements and large areas of secretory material in the intervening areas, suggestive of cellular PA [Figure 3]. However since most of the tumors of submandibular and sublingual gland are malignant, a second examination of the specimen was undertaken. It showed that the entire tumor mass was covered by dense band of fibrous connective tissue. Tumor cells were arranged in a background of predominantly mucous acini, as small tubules lined by cuboidal to oval cells containing prominent nuclei and eosinophilic cytoplasm and surrounded by clear cells and increased hyalinization [Figure 4]. On the basis of this dual cell population, a provisional diagnosis of EMC was suggested. Some focal areas were also observed with cells arranged in a cribriform pattern with pseudocystic spaces that pointed toward Ad CC. Immunohistochemistry showed p-63 reactivity in outer clear cells, S-100 and smooth muscle actin were focally positive and C-kit for Ad CC was negative. Hence, a diagnosis of hybrid carcinoma was ruled out and a final diagnosis of epi-myoepithelial carcinoma was made. There is a chance that being a long-standing tumor; initial PA may have turned into EMC. Patient denied adjuvant Radiotherapy; is being followed-up since last 1 year and has no fresh complains or signs of recurrence until now [Figure 5].
Figure 1: Clinical picture

Click here to view
Figure 2: Magnetic resonance imaging of the lesion

Click here to view
Figure 3: H and E section suggestive of cellular pleomorphic adenoma

Click here to view
Figure 4: H and E section suggestive of epithelial-myoepithelial carcinoma

Click here to view
Figure 5: Post-operative clinical picture

Click here to view



   Discussion Top


EMC was first described by Donath et al., in 1972 and officially defined as a salivary gland tumor by WHO in 1991. It commonly affects parotid gland (70%), sino-nasal glands, palatal and submandibular gland and rarer sites like lungs, breasts etc., (no reports on sublingual gland origin were found). It is a low-grade carcinoma of ductal origin characterized by its dual cell population of ductal epithelial cells and clear myoepithelial cells. Apart from this characteristic picture, there are many histological variants such as oncocytic EMC, double clear EMC, EMC ex PA, high grade or dedifferentiated EMC, EMC with myoepithelial anaplasia etc. [1],[4] The median age of occurrence is 60.4 years. [1],[4] Many times, it is found that pre-operative FNAC points toward a benign pathology (PA). [5],[6] A diagnosis of PA would be incorrect due to the excessive hyalinization seen in the tissue, in spite of the pleomorphic picture of cells. Differential diagnosis includes other clear cell tumors such as muco-epidermoid carcinoma, acinic cell carcinoma and sebaceous carcinoma, with immunohistochemical presence of myoepithelial markers, similar to those seen in EMC. However they lack the biphasic pattern. Furthermore, there have been reports describing EMC ex PA in parotid gland with the histological picture similar to ours. [2] Within the characteristic dual cell picture of EMC, arrangements of cells in cribriform pattern with pseudocystic spaces reminiscent of Ad CC were observed focally. Woo et al., have suggested such an overlap in previous literature. In the presenting case, the tumor could have been a primary onset EMC or an EMC-ex-PA. There have been cases of recurrence of tumor by simple excision alone, [1],[3],[7] hence we chose wider margins of healthy mucosa. The prognosis of EMC is fairly good as the median disease free survival rate is reported to be 11.34 years. [1] Long-term follow-up is needed for detection of early signs of recurrence and proper management.

 
   References Top

1.
Brocheriou C, Auriol M, de Roquancourt A, Gaulard P, Fornes P. Epithelial-myoepithelial carcinoma of the salivary glands. Study of 15 cases and review of the literature. Ann Pathol 1991;11:316-25.  Back to cited text no. 1
    
2.
Kainuma K, Oshima A, Suzuki H, Fukushima M, Shimojo H, Usami S. Hybrid carcinoma of the parotid gland: Report of a case (epithelial-myoepithelial carcinoma and salivary duct carcinoma) and review of the literature. Acta Otolaryngol 2010;130:185-9.  Back to cited text no. 2
    
3.
Daneshbod Y, Negahban S, Khademi B, Daneshbod K. Epithelial myoepithelial carcinoma of the parotid gland with malignant ductal and myoepithelial components arising in a pleomorphic adenoma: A case report with cytologic, histologic and immunohistochemical correlation. Acta Cytol 2007;51:807-13.  Back to cited text no. 3
    
4.
Seethala RR, Barnes EL, Hunt JL. Epithelial-myoepithelial carcinoma: A review of the clinicopathologic spectrum and immunophenotypic characteristics in 61 tumors of the salivary glands and upper aerodigestive tract. Am J Surg Pathol 2007;31:44-57.  Back to cited text no. 4
    
5.
Mahapatra S. Epithelial-Myoepithelial Carcinoma of Parotid - A Rare Case Report. Webmedcentral Pathol 2011;2:WMC002566.  Back to cited text no. 5
    
6.
Arora SK, Sharma N. Epithelial myoepithelial carcinoma of head and neck region. Indian J Otolaryngol Head Neck Surg 2013;65:163-6.  Back to cited text no. 6
    
7.
Lokuhetty MD, Premathilake IV, Amarasinghe C. Epimyoepithelial carcinoma - An uncommon salivary gland tumor. J Diagn Pathol 2002-2003;1:25-7.  Back to cited text no. 7
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]


This article has been cited by
1 NUT Midline Carcinoma of the Sublingual Gland: Clinical Presentation and Review
Nolan B. Seim,Ramez H. W. Philips,Lynn Schoenfield,Theodoros N. Teknos,James W. Rocco,Amit Agrawal,Enver Ozer,Ricardo L. Carrau,Stephen Y. Kang,Matthew O. Old
Head and Neck Pathology. 2017;
[Pubmed] | [DOI]
2 Epithelial myoepithelial carcinoma of minor salivary gland A case report
Basudev Mahato,Samir Mandal,Tushar Deb,Jay Gopal Ray,Keya Chaudhuri
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. 2016;
[Pubmed] | [DOI]
3 Epithelial-myoepithelial carcinoma arising in the oral floor: Report of a case and review of the literature
Masakatsu Fukuda,Kentaro Kikuchi,Kaoru Kusama,Hideaki Sakashita
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. 2016;
[Pubmed] | [DOI]



 

Top
   
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
   Case Report
   Discussion
    References
    Article Figures

 Article Access Statistics
    Viewed1103    
    Printed27    
    Emailed0    
    PDF Downloaded218    
    Comments [Add]    
    Cited by others 3    

Recommend this journal